Thursday, 15 June 2017

The Comparative Study of Myofibrillar Proteins of Skeletal Muscles of Some Deep-Sea Fish Species

Deep-sea fish have not generally been used for food, but its abundance suggests it could be an important source of highquality protein. Deep-sea fish have much different significance for industrial fishing. Three species of deep-sea fish Coryphaenoides pectoralis, Coryphaenoides cinereus and Podonema longipes are the most accessible for effective fishing in the Far-Eastern Seas of Russia. 
These three species were chosen for investigations in the present paper.For processing of fish meat, the knowledge of the properties of fish muscle proteins is necessary. The main aim of the present paper was to determine the composition of basic myofibrillar proteins and its ratio in skeletal muscle tissue.

Investigations of the biochemistry of muscle tissue of deepsea fish have only incidental character and systematic study of this has not been carried out. In most cases the studies of researchers have been limited to mesopelagic species.


Monday, 5 June 2017

Selection of Reliable Reference Genes for Quantitative Real-Time PCR in Golden-Line Barbell (Sinocyclocheilus grahami) During Juvenile and Adult Stages

In order to obtain reliable results of gene expression, appropriate internal control gene(s) were required for normalization before real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).
Reliable Reference Genes

Here, we selected eight candidate housekeeping genes as the potential reference genes in normalizing qRT-PCR data in ten tissues of the juvenile and adult stages of a naturally tetraploid cyprinid fish Sinocyclocheilus grahami.

Before qRT-PCR, appropriate primers were designed and compared for distinguishing different copies in some duplicate genes. Candidate reference genes were evaluated for their stability using online software RefFinder, which is integrated by four normal software of the comparative delta-CT, BestKeeper, NormFinder and GeNorm.

Tuesday, 30 May 2017

Survey on Prevalence of Newcastle Disease Antibodies in Village Poultry at Live Birds Markets in Gombe, Nigeria

Newcastle Disease The study aimed to determine the seroprevalence of Newcastle disease (ND) amongst some village poultry species at poultry markets in Gombe, Nigeria.

A total of 1200 (841 Village chickens, 320 Guinea Fowls and 39 Pigeons) sera samples were tested. Haemagglutination inhibition test revealed an overall ND virus (NDV) antibodies prevalent rate of 53.7% (644/1200).

The seroprevalence of antibodies to ND was found to be higher in Village chickens 527/841 (62.7%) followed by Pigeons 19/39 (48.7%) and Guinea Fowls 98/320 (30.6%) respectively and were found to be seropositive with Geometric Mean Titres (GMT) of 1.9 to 5.9.

Wednesday, 24 May 2017

Analysis of the Gut Microbiome of Nile Tilapia Oreochromis Niloticus L. Fed Diets Supplemented with Previda® and Saponin

The use of prebiotics in aquaculture aims at increasing the numbers of beneficial microbes in the gastrointestinal tract of fishes.

However, the true effect of prebiotics exert on the fish microbiome is for the most part unknown.
Microbiome of Nile

This study aimed at investigating the effects of the commercial prebiotic Previda® and the feed supplement saponin on Nile tilapia (Oreochromis niloticus L.).

Monday, 15 May 2017

Welfare Indicators of Milking Sheep and Goats in Commercial Farms in Spain

The objective of this work was to evaluate a system for assessing the welfare of milking sheep and goats in practical farm conditions.

The system uses indicators of environmental, management and facilities related welfare. In order to evaluate the animal welfare, 12 indicators ranked in 3 groups were defined.
Commercial Farms in Spain

Environment Animal Welfare (EAW, n = 4) (temperature, airflow, ventilation and ammonia load) Management Animal Welfare (MAW, n = 5) (animal load, bedding, comfort, stress and cleanness of animals) and Facilities Animal Welfare (FAW, n = 3) (feeders of concentrate, feeders of forage and watering).

Monday, 8 May 2017

Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress

Xenobiotics are synthetic compounds foreign to the biological system. They often retain their qualities in the aquatic environment with the ability to cause oxidative stress in these organisms by activating the endogenous production of reactive oxygen species (ROS).
Xenobiotics are synthetic compounds foreign to the biological system. They often retain their qualities in the aquatic environment with the ability to cause oxidative stress in these organisms by activating the endogenous production of reactive oxygen species (ROS).  Overloading of the estuaries with contaminants for a longer period has an impact on fish production. The grey mullet (Mugil cephalus) is capable of concentrating contaminants and is considered suitable for biomarker studies.  Brain is an appropriate organ for the study of the effects of xenobiotics due to its morphological heterogeneity, metal accumulating capacity and susceptibility to histopathological damage by metals.  PDF LINK

Overloading of the estuaries with contaminants for a longer period has an impact on fish production. The grey mullet (Mugil cephalus) is capable of concentrating contaminants and is considered suitable for biomarker studies.

Brain is an appropriate organ for the study of the effects of xenobiotics due to its morphological heterogeneity, metal accumulating capacity and susceptibility to histopathological damage by metals.

Tuesday, 2 May 2017

Fish in super-shops: A new dimension of fish marketing system in Dhaka

In this study, fish marketing system of four affluent super-shops and 8 outlets selling wet fishes (2 outlets for each shop) in Dhaka (Bangladesh) were investigated.
super-shops

It was observed that a significant portion of the fishes were supplied from different wholesale fish markets of Dhaka metropolitan city and fish production zones of adjacent districts.

Various stakeholders were involved in fish value chain concerning super shop outlets. The availability of fish was more or less similar in all super shops. Three types of supply chains were identified as selling fish through super shops.