In order to
obtain reliable results of gene expression, appropriate internal control
gene(s) were required for normalization before real-time quantitative reverse
transcription polymerase chain reaction (qRT-PCR).
Here, we selected eight
candidate housekeeping genes as the potential
reference genes in normalizing qRT-PCR data in ten tissues of the juvenile and
adult stages of a naturally tetraploid cyprinid fish Sinocyclocheilus grahami.
Before qRT-PCR,
appropriate primers were designed and compared for distinguishing different
copies in some duplicate genes. Candidate reference genes were evaluated for
their stability using online software RefFinder, which is integrated by four
normal software of the comparative delta-CT, BestKeeper, NormFinder and GeNorm.
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