Genetically modified animals, in particular
mice, are unique mutants with a major scientific potential. Cryopreservation of
pre-implantation
embryos or of spermatozoa is a common approach to protect those
lines against loss or to archive them.
Prerequisite to take a mutant line out of a
breeding nucleus is the cryopreservation of a sufficient number of samples and
a powerful quality assessment.
Several common protocols to cryopreserve
spermatozoa and subsequently to forward these to an in vitro-fertilization
under standardized conditions are published using donors of a strictly limited
age-span.
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