Several common
protocols to cryopreserve spermatozoa and subsequently to forward these to an
in vitro-fertilization under standardized conditions are published using donors
of a strictly limited age-span.
In practice, several
limitations happen frequently for those approaches, e.g. the
availability of genetically modified males, in particular if a line is in an
experimental use or the mutation leads to restrictions.
Female donors are
often received from external sources and are subsequently not available before
weaning. They are subject of several delays to induce an early superovulation.
The aim of this report is to study the suitable age span of sperm and oocyte
donors.
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